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KMID : 1161420130160110997
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Journal of Medicinal Food
2013 Volume.16 No. 11 p.997 ~ p.1003
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Inhibitory Effects of Salidroside on Nitric Oxide and Prostaglandin E2 Production in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages
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Song Bocui
Huang Guoren
Xiong Ying
Liu Jingbo
Xu Linli
Wang Zhenning
Li Gen
Lu Jing
Guan Shuang
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Abstract
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The aim of this study was to evaluate the effect of salidroside on lipopolysaccharide (LPS)-induced nitric oxide (NO) and prostaglandin E2 (PGE2) production in RAW 264.7 macrophages and related anti-inflammatory mechanism. PGE2 production was measured by enzyme-linked immunosorbent assay (ELISA); NO production was tested by Griess reagent. Inducible nitric oxidesynthase (iNOS) and COX-2 were determined by RT-PCR and Western blot analysis; IκB and P-IκB protein express were detected by Western blot analysis; cytosolic free Ca2+ ([Ca2+]i)was measured by a fluorescent microscope. The data showed salidroside inhibited LPS-induced NO and PGE2 production and reduced iNOS and COX-2 protein expression in RAW 264.7 macrophages. Consistent with these observations, salidroside inhibited LPS-induced cytosolic free Ca2+ concentration ([Ca2+]i) elevation. In addition, we further investigated signal transduction mechanisms and found that the activation of NF-κB was suppressed by salidroside in a dose-dependent manner. These results suggest that salidroside suppresses NO and PGE2 production by inhibiting iNOS and COX-2 protein expression, level of [Ca2+]i, and activation of NF-κB signal transduction pathway.
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KEYWORD
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[Ca2+]i, COX-2, iNOS, NF-κB, NO, PGE2, salidroside
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